Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities

Bioprocess Biosyst Eng. 2020 Nov;43(11):2017-2026. doi: 10.1007/s00449-020-02390-w. Epub 2020 Jun 22.

Abstract

Herein, we reported a green biosynthesis method of copper nanoparticles (CuNPs) at microwave irradiation condition by using pectin as a stabilizer and ascorbic acid as a reducing agent. Under the optimum conditions, CuNPs1 and 2 were synthesized under microwave times 0 and 3 min, respectively. Transmission electron microscope and scanning electron microscope (SEM) tests showed that CuNPs1 and 2 had irregular polygon particles with average diameters of 61.9 ± 19.4 and 40.9 ± 13.6 nm, respectively. Zeta potentials of CuNPs1 and 2 were -45.2 and -48.7 mV, respectively. X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and X-ray photoelectron spectroscopy techniques were used to characterize the properties of CuNPs. Furthermore, inhibition zone tests showed that CuNPs2 exhibited higher antimicrobial activities against Escherichia coli, Staphylococcus aureus, and Aspergillus japonicus than CuNPs1. The antibacterial activities were also studied by the bacterial growth kinetics in broth media, and CuNPs2 exhibited lower minimum bactericidal concentrations than CuNPs1.

Keywords: Antimicrobial activities; Copper nanoparticles; Microwave; Pectin.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Anti-Infective Agents / chemical synthesis*
  • Ascorbic Acid
  • Aspergillus / drug effects
  • Copper / chemistry*
  • Escherichia coli / drug effects
  • Green Chemistry Technology
  • Kinetics
  • Metal Nanoparticles / chemistry*
  • Microbial Sensitivity Tests*
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Particle Size
  • Pectins / chemistry*
  • Photoelectron Spectroscopy
  • Spectroscopy, Fourier Transform Infrared
  • Staphylococcus aureus / drug effects
  • Thermogravimetry
  • X-Ray Diffraction

Substances

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Copper
  • Pectins
  • Ascorbic Acid

Supplementary concepts

  • Aspergillus japonicus