We report a biosensing platform based on a binary mixture comprised of Au nanorods (plasmonic nanoparticles, Au NRs) and magnetically responsive Pt@Ni nanorings (magnetic nanostirrers, MN-rings). The mixture of Au NRs and MN-rings was modulated with an external rotating magnetic field (a dynamic assay with magnetic perturbation), which led to fluctuating extinction in the UV-vis spectroscopy measurement. As the surfaces of Au NRs were modified with antigens and antibodies, their periodic profile of extinction changed in accordance with surface modification of the Au NRs. The obtained periodic extinction with time could be converted to a frequency domain function where the signal-to-noise ratios of the peaks were evaluated to monitor surface biorecognitions on Au NRs, which is in contrast to conventional biosensors (a stagnant assay without perturbation) that use only the peak shift of localized surface plasmon resonance of Au nanoparticles.