Abstract
Normal human T cells purified from the peripheral blood and cultured for 6 days in the presence of OKT3 and IL-2 were shown to produce simultaneously TNF and lymphotoxin (LT) mRNA. Both biologically active TNF and LT were detected in supernatants of the cultured T cells using WEHI 164 as target cells. The cytotoxic activities of both cytokines could be inhibited with specific antibodies against TNF or LT. The T cell cultures were free of macrophages, NK cells, or B lymphocytes as assessed by flow cytometric analysis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal / immunology
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Antigens, Differentiation, T-Lymphocyte / immunology
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Cell Separation
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Cells, Cultured
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Humans
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Interleukin-2 / pharmacology
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Lymphocyte Activation
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Lymphotoxin-alpha / biosynthesis*
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T-Lymphocytes / drug effects
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T-Lymphocytes / metabolism*
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Tumor Necrosis Factor-alpha / biosynthesis*
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation, T-Lymphocyte
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Interleukin-2
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Lymphotoxin-alpha
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Tumor Necrosis Factor-alpha