CD8+CD28null T Lymphocytes are Associated with the Development of Atrial Fibrillation after Elective Cardiac Surgery

Thromb Haemost. 2020 Aug;120(8):1182-1187. doi: 10.1055/s-0040-1713096. Epub 2020 Jun 28.

Abstract

Background: Postoperative atrial fibrillation (POAF) is assumed as a complex and multifactorial interaction of different pathogenic factors. Data suggests an inflammatory process as the main trigger of this specific type of atrial fibrillation. CD8+ T lymphocytes that lack the surface protein CD28 were found to be crucially involved in chronic inflammatory processes within the cardiovascular system. Of utmost interest, these so-called CD8+CD28null T cells are known to present with autoaggressive behavior and deleterious cytotoxic effects on human tissue.

Methods: A total of 129 patients undergoing elective cardiac valve and/or coronary artery bypass graft surgery were enrolled. Fluorescence-activated cell sorting was performed to investigate lymphocyte subsets. Patients were stratified in two subgroups according to patients developing POAF (n = 60) and individuals free of POAF (n = 69).

Results: Comparing patients developing POAF to individuals free of POAF, the fraction of CD8+ lymphocytes was significantly higher in individuals developing POAF (30.5% [POAF] vs. 25.7% [no-POAF]; p = 0.021). Interestingly, also the fraction of CD8+CD28null T lymphocytes was significantly higher in the POAF subgroup (66.7% [POAF] vs. 61.6% [non-POAF]; p = 0.043). Multivariate logistic regression proved that the fraction of CD8+CD28null cells is a strong and independent prognosticator for the development of POAF with an adjusted odds ratio per 1 standard deviation of 3.21 (95% confidence interval 1.01-10.18; p = 0.048).

Conclusion: We found that cytotoxic CD8+CD28null T lymphocytes proved to be a strong and independent predictor for the development of POAF after elective cardiac surgery. Our results potentially indicate an autoimmune impact of this preexisting, highly cytotoxic T cell subset in the pathogenesis of POAF.

Publication types

  • Observational Study

MeSH terms

  • Aged
  • Atrial Fibrillation / etiology*
  • Atrial Fibrillation / immunology
  • C-Reactive Protein / analysis
  • CD28 Antigens / analysis
  • Comorbidity
  • Coronary Artery Bypass*
  • Cytotoxicity, Immunologic
  • Elective Surgical Procedures*
  • Female
  • Heart Valve Prosthesis Implantation*
  • Humans
  • Inflammation
  • Male
  • Middle Aged
  • Postoperative Complications / etiology*
  • Postoperative Complications / immunology
  • Prospective Studies
  • T-Lymphocyte Subsets / immunology*

Substances

  • CD28 Antigens
  • C-Reactive Protein