Abstract
The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on serotonin-induced inositol phosphate (IP) accumulation and intracellular free Ca2+ concentrations [( Ca2+]i) was investigated in cultured rat vascular smooth muscle cells. Pretreatment with TPA had no effect on basal levels of both IP production and [Ca2+]i, whereas it significantly attenuated serotonin-induced increases in both IP production and [Ca2+]i. These data suggest that protein kinase C is involved in the negative feedback control of serotonin-induced rises in both IP production and [Ca2+]i.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Benzofurans
-
Calcium / metabolism*
-
Cells, Cultured
-
Fluorescent Dyes
-
Fura-2
-
Inositol 1,4,5-Trisphosphate
-
Inositol Phosphates / biosynthesis*
-
Ketanserin / pharmacology
-
Male
-
Muscle, Smooth, Vascular / drug effects
-
Muscle, Smooth, Vascular / metabolism*
-
Protein Kinase C / metabolism
-
Rats
-
Rats, Inbred Strains
-
Receptors, Serotonin / drug effects
-
Receptors, Serotonin / physiology*
-
Serotonin / pharmacology
-
Sugar Phosphates / biosynthesis*
-
Tetradecanoylphorbol Acetate / pharmacology*
Substances
-
Benzofurans
-
Fluorescent Dyes
-
Inositol Phosphates
-
Receptors, Serotonin
-
Sugar Phosphates
-
Serotonin
-
Inositol 1,4,5-Trisphosphate
-
Ketanserin
-
Protein Kinase C
-
Tetradecanoylphorbol Acetate
-
Calcium
-
Fura-2