A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

Methods Mol Biol. 2020:2156:187-202. doi: 10.1007/978-1-0716-0660-5_14.

Abstract

Lipid changes that occur in leaves of plants (e.g., Arabidopsis thaliana), during cold and freezing stress can be analyzed with electrospray ionization triple quadrupole mass spectrometry, using high-throughput multiple reaction monitoring (MRM). An online tool, LipidomeDB Data Calculation Environment, is employed for mass spectral data processing.

Keywords: Cold; Freezing; Lipidomics; Lipidomics data processing; Mass spectrometry; Multiple reaction monitoring (MRM); Postfreezing recovery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acclimatization
  • Arabidopsis / physiology*
  • Cold-Shock Response*
  • Data Analysis
  • Freezing*
  • Lipid Metabolism*
  • Lipidomics* / methods
  • Membrane Lipids / metabolism*
  • Phenotype
  • Plant Physiological Phenomena

Substances

  • Membrane Lipids