Clonal analysis of human T cell activation by the Mycoplasma arthritidis mitogen (MAS)

Eur J Immunol. 1988 Nov;18(11):1733-7. doi: 10.1002/eji.1830181112.

Abstract

Mycoplasma arthritidis produces an as yet undefined soluble molecule (MAS) that has a potent mitogenic effect on T cells of several species. We have used cloned human cytotoxic and proliferative T lymphocytes to dissect the molecular mechanism of T cell activation by this mitogen. Reactivity to MAS is clonally expressed among T cell receptor (TcR) alpha/beta chain-expressing T cell clones of CD4+ or CD8+ phenotype, as well as CD4-8- TcR alpha/beta chain-negative T lymphocyte clones expressing the CD3-associated TcR gamma chain. MAS is able to induce cytotoxicity and/or proliferation in these T cell clones. For triggering of these T cells, regardless of their phenotype of specificity, the presence of autologous, allogeneic or xenogeneic major histocompatibility complex (MHC) class II molecules on accessory cells or target cells is necessary. However, T cells do not immunologically recognize MAS on class II molecules, since a direct action of MAS on the T cells themselves can be demonstrated. Triggering of T cells by MAS can be blocked by monoclonal antibodies against CD2, CD3 and the TcR alpha/beta chain dimer. We discuss as a possible explanation that MAS is a functionally bivalent molecule cross-linking TcR and MHC class II molecules. Thus, the mechanism of T cell activation by MAS has striking similarities to the mechanisms by which Staphylococcal enterotoxins activate T cells. It is intriguing that a similar mitogenic principle has been developed by two evolutionary distinct pathogenic microorganisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen-Antibody Reactions
  • Antigens, Bacterial / immunology*
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • Clone Cells
  • Cytotoxicity, Immunologic*
  • HLA-D Antigens / immunology
  • Humans
  • In Vitro Techniques
  • Lymphocyte Activation*
  • Mycoplasma / immunology*
  • Receptors, Antigen, T-Cell / immunology
  • Species Specificity
  • T-Lymphocytes / immunology*

Substances

  • Antigens, Bacterial
  • Antigens, Differentiation, T-Lymphocyte
  • HLA-D Antigens
  • Receptors, Antigen, T-Cell