Comparative Genomics of a Paddy Field Bacterial Isolate Ochrobactrum sp. CPD-03: Analysis of Chlorpyrifos Degradation Potential

Tanmaya Nayak; Ananta N Panda; Khushbu Kumari; Tapan Kumar Adhya; Vishakha Raina

doi:10.1007/s12088-020-00864-9

Comparative Genomics of a Paddy Field Bacterial Isolate Ochrobactrum sp. CPD-03: Analysis of Chlorpyrifos Degradation Potential

Indian J Microbiol. 2020 Sep;60(3):325-333. doi: 10.1007/s12088-020-00864-9. Epub 2020 Apr 2.

Authors

Tanmaya Nayak¹, Ananta N Panda¹, Khushbu Kumari¹, Tapan Kumar Adhya¹, Vishakha Raina¹

Affiliation

¹ Environmental Biotechnology Laboratory, School of Biotechnology, Kalinga Institute of Industrial Technology-KIIT (Deemed to be University), Bhubaneswar, Odisha 751024 India.

Abstract

Ochrobactrum genus is known to catabolize aromatic compounds. This study reports a complete genome sequence of Ochrobactrum sp. CPD-03 (~ 4.6 Mb of chromosomal features) responsible for chlorpyrifos (CP) isolated form a paddy field (20.3588° N, 85.8333° E) in Bhubaneswar, India. A comparative genomics approach was performed between CPD-03 and eight closely related genomes of other Ochrobactrum strains in order to deepen our knowledge, to establish its phylogenetic and functional relationships. The involvement of CP degrading genes indicated a versatile role of CPD-03 in additional field trails. This research would provide the genetic information for its use in natural environment for the depletion of organophosphorus (OP) compounds.

Keywords: Arylesterase; Chlorpyrifos; Ochrobactrum sp. CPD-03; Phylogenomics; Xenobiotic compound degradation.