Corilagin suppresses RANKL-induced osteoclastogenesis and inhibits oestrogen deficiency-induced bone loss via the NF-κB and PI3K/AKT signalling pathways

J Cell Mol Med. 2020 Sep;24(18):10444-10457. doi: 10.1111/jcmm.15657. Epub 2020 Jul 18.

Abstract

Over-activated osteoclastogenesis, which is initiated by inflammation, has been implicated in osteoporosis. Corilagin, a natural compound extracted from various medicinal herbaceous plants, such as Cinnamomum cassia, has antioxidant and anti-inflammatory activities. We found that Corilagin suppressed osteoclast differentiation in a dose-dependent manner, significantly decreased osteoclast-related gene expression and impaired bone resorption by osteoclasts. Moreover, phosphorylation of members of the nuclear factor-kappaB (NF-κB) and PI3K/AKT signalling pathways was reduced by Corilagin. In a murine model of osteoporosis, Corilagin inhibited osteoclast functions in vivo and restored oestrogen deficiency-induced bone loss. In conclusion, our findings suggested that Corilagin inhibited osteoclastogenesis by down-regulating the NF-κB and PI3K/AKT signalling pathways, thus showing its potential possibility for the treatment of osteoporosis.

Keywords: Corilagin; NF-κB; PI3K/AKT; RANKL; osteoclast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / metabolism
  • Bone Resorption / pathology*
  • Down-Regulation / drug effects
  • Estrogens / deficiency*
  • Glucosides / chemistry
  • Glucosides / pharmacology*
  • Hydrolyzable Tannins / chemistry
  • Hydrolyzable Tannins / pharmacology*
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / metabolism*
  • NFATC Transcription Factors / metabolism
  • Osteoclasts / drug effects
  • Osteoclasts / metabolism
  • Osteoclasts / pathology
  • Osteogenesis / drug effects*
  • Osteoprotegerin / metabolism
  • Ovariectomy
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Proto-Oncogene Proteins c-akt / metabolism*
  • RANK Ligand / pharmacology*
  • RAW 264.7 Cells
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects

Substances

  • Actins
  • Estrogens
  • Glucosides
  • Hydrolyzable Tannins
  • NF-kappa B
  • NFATC Transcription Factors
  • Osteoprotegerin
  • RANK Ligand
  • RNA, Messenger
  • Reactive Oxygen Species
  • corilagin
  • Proto-Oncogene Proteins c-akt