Electrochemical aptasensor using boron-carbon nanorods decorated by nickel nanoparticles for detection of E. coli O157:H7

Mikrochim Acta. 2020 Jul 20;187(8):461. doi: 10.1007/s00604-020-04444-y.

Abstract

The development of a label-free impedimetric aptasensor is reported for rapid and sensitive detection of Escherichia coli O157:H7 employing boron-carbon nanorods decorated by nickel nanoparticles (BC-Ni) nanostructured platform. These highly electroactive BC-Ni nanorods were synthesized to increase the sensitivity of the sensor surface and subsequently functionalized with a specific anti-E. coli O157:H7 aptamer (Kd = 69 nM) as bio-recognition moiety. This fully characterized high-affinity DNA aptamer against the bacteria was selected using a facile microtiter plate-based cell-SELEX methodology. The fabricated electrochemical aptasensor is demonstrated to detect E. coli O157:H7 selectively with a detection limit of 10 cfu and a dynamic detection range of 100 to 105 cfu in water, juice, and fecal samples. Graphical abstract.

Keywords: Aptasensor; Boron-carbon nanostructures; E. coli O157:H7; Nanosensor; Pathogen detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Base Sequence
  • Biosensing Techniques / methods*
  • Boron / chemistry
  • Carbon / chemistry
  • DNA / chemistry
  • Drinking Water / microbiology
  • Electrochemical Techniques / methods
  • Escherichia coli O157 / chemistry
  • Escherichia coli O157 / isolation & purification*
  • Feces / microbiology
  • Fruit and Vegetable Juices / microbiology
  • Metal Nanoparticles / chemistry*
  • Nanotubes / chemistry*
  • Nickel / chemistry
  • SELEX Aptamer Technique

Substances

  • Aptamers, Nucleotide
  • Drinking Water
  • Carbon
  • Nickel
  • DNA
  • Boron