Microbial biodiversity in the throats of pulmonary tuberculosis patients and tuberculin skin test (TST) positive and negative healthy individuals in Malaysia

Noreafifah Semail; Siti Suraiya; Romel Calero; Mayelin Mirabal; Humberto Carrillo; Mohamed Hashim Ezzeddin Kamil; Maria E Sarmiento; Armando Acosta; Mohd Nor Norazmi

doi:10.1016/j.tube.2020.101965

Microbial biodiversity in the throats of pulmonary tuberculosis patients and tuberculin skin test (TST) positive and negative healthy individuals in Malaysia

Tuberculosis (Edinb). 2020 Sep:124:101965. doi: 10.1016/j.tube.2020.101965. Epub 2020 Jul 12.

Authors

Noreafifah Semail¹, Siti Suraiya², Romel Calero³, Mayelin Mirabal⁴, Humberto Carrillo⁵, Mohamed Hashim Ezzeddin Kamil⁶, Maria E Sarmiento⁷, Armando Acosta⁸, Mohd Nor Norazmi⁹

Affiliations

¹ School of Medical Sciences, Universiti Sains Malaysia (USM), 16150, Kubang Kerian, Kelantan, Malaysia. Electronic address: [email protected].
² School of Medical Sciences, Universiti Sains Malaysia (USM), 16150, Kubang Kerian, Kelantan, Malaysia. Electronic address: [email protected].
³ Center of Complexity Science, National Autonomous University of Mexico (UNAM), Mexico. Electronic address: [email protected].
⁴ Center of Complexity Science, National Autonomous University of Mexico (UNAM), Mexico; Institute of Nuclear Sciences, National Autonomous University of Mexico, Mexico. Electronic address: [email protected].
⁵ Center of Complexity Science, National Autonomous University of Mexico (UNAM), Mexico; Faculty of Science, National Autonomous University of Mexico (UNAM), Mexico. Electronic address: [email protected].
⁶ School of Health Sciences, Universiti Sains Malaysia (USM), 16150, Kubang Kerian, Kelantan, Malaysia. Electronic address: [email protected].
⁷ School of Health Sciences, Universiti Sains Malaysia (USM), 16150, Kubang Kerian, Kelantan, Malaysia. Electronic address: [email protected].
⁸ School of Health Sciences, Universiti Sains Malaysia (USM), 16150, Kubang Kerian, Kelantan, Malaysia. Electronic address: [email protected].
⁹ School of Health Sciences, Universiti Sains Malaysia (USM), 16150, Kubang Kerian, Kelantan, Malaysia. Electronic address: [email protected].

PMID: 32692651
DOI: 10.1016/j.tube.2020.101965

Abstract

The purpose of this study was to investigate the composition of throat microbiota in pulmonary tuberculosis patients (PTB) in comparison to healthy tuberculin skin test positive (TSTp) and negative (TSTn) individuals. Throat swabs samples were collected, and the microbiota was characterized. Richer operational taxonomic units (OTUs) were present in PTB group, compared to TSTp and TSTn. Regarding alpha diversity analysis there was a higher community diversity in TSTn compared to TSTp. Beta diversity analysis showed different species composition in TSTp compared to TSTn and PTB. There was higher presence of Firmicutes in PTB and TSTn compared to TSTp group at phylum level. At the genus level, Leuconostoc and Enterococcus were higher in TSTn compared to TSTp and Pediococcus, Chryseobacterium, Bifidobacterium, Butyrivibrio, and Bulleidia were higher in PTB compared to TSTn. Streptococcus was higher in TSTn compared to PTB and Lactobacillus in PTB compared to TSTp. At species level, Streptococcus sobrinus and Bulleidia moorei were higher in PTB compared to TSTn individuals, while Lactobacillus salivarius was higher in PTB compared to TSTp. The differences in the microbiome composition could influence the resistance/susceptibility to Mtb infection.

Keywords: Latent tuberculosis; Metagenomic; Microbiome; Pulmonary tuberculosis; Throat swabs; Tuberculin skin test.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / classification
Bacteria / genetics
Bacteria / isolation & purification*
Case-Control Studies
Humans
Malaysia
Microbiota*
Pharynx / microbiology*
Predictive Value of Tests
Ribotyping
Tuberculin Test*
Tuberculosis, Pulmonary / diagnosis
Tuberculosis, Pulmonary / microbiology*