Objective: To investigate the potential allergenicity of oryza sativa recombinant human serum albumin(OsrHSA)in BALB/c mice.
Methods: Eighty BALB/c mice were randomly divided into four groups i. e ovalbumin(OVA) positive control group, potato acid phosphatase(PAP) negative control group, Oryza sativa recombinant HAS(OsrHSA) group and solvent control group(phosphate buffer saline, PBS), respectively. Mice were administered by intraperitoneal injections of tested proteins and histamine levels in plasma and sIgE, sIgG, sIgG1, sIgG2 a, and tIgE antibody levels in serum were measured.
Results: Compared with the other groups, serum tIgE, sIgE, sIgG, sIgG1 and plasma histamine levels in the OVA group were significantly increased, while serum sIgG2 a levels were decreased(P<0. 05). There was no significant difference in serum sIgE level and histamine level between the OsrHSA group and the control group(P>0. 05). Serum sIgG, sIgG1 and sIgG2 a levels were lower than those in the PAP group(P<0. 05). There was no significant difference in serum tIgE content between PAP group and OsrHSA group(P>0. 05).
Conclusion: The potential allergenicity of OsrHSA through traperitioneal injection in BALB/c mice was very low.
Keywords: BALB/c mice; allergenicity; food safety; genetically modified food; human serum albumin.