1H, 13C and 15N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei

Biomol NMR Assign. 2020 Oct;14(2):309-315. doi: 10.1007/s12104-020-09968-1. Epub 2020 Jul 21.

Abstract

KKT4 is a kinetoplastid-specific microtubule-binding kinetochore protein that lacks significant similarity to any known kinetochore or microtubule-binding proteins. Here we present the 1H, 13C and 15N resonance assignments for several fragments from the microtubule-binding domain of KKT4 (KKT4115-343) from Trypanosoma brucei. These assignments provide the starting point for detailed investigations of the structure, dynamics and interactions of the microtubule-binding region of KKT4.

Keywords: KKT4; Kinetochore; Kinetoplastid; NMR resonance assignments; Trypanosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon-13 Magnetic Resonance Spectroscopy*
  • Kinetochores / metabolism*
  • Microtubules / metabolism*
  • Nitrogen Isotopes
  • Protein Domains
  • Proton Magnetic Resonance Spectroscopy*
  • Protozoan Proteins / analysis*
  • Protozoan Proteins / chemistry*
  • Trypanosoma brucei brucei / metabolism*

Substances

  • Nitrogen Isotopes
  • Nitrogen-15
  • Protozoan Proteins