Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum

Shuen Hon; Liang Tian; Tianyong Zheng; Jingxuan Cui; Lee R Lynd; Daniel G Olson

doi:10.1007/978-1-0716-0195-2_3

Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum

Methods Mol Biol. 2020:2096:21-43. doi: 10.1007/978-1-0716-0195-2_3.

Authors

Shuen Hon^{1

2}, Liang Tian^{1

2}, Tianyong Zheng^{1

2}, Jingxuan Cui^{1

2}, Lee R Lynd^{1

2}, Daniel G Olson^{3

4}

Affiliations

¹ Thayer School of Engineering at Dartmouth, Hanover, NH, USA.
² Center for Bioenergy Innovation, Oak Ridge, TN, USA.
³ Thayer School of Engineering at Dartmouth, Hanover, NH, USA. [email protected].
⁴ Center for Bioenergy Innovation, Oak Ridge, TN, USA. [email protected].

PMID: 32720144
DOI: 10.1007/978-1-0716-0195-2_3

Abstract

In this work, we describe genetic tools and techniques for engineering Thermoanaerobacterium saccharolyticum. In particular, the T. saccharolyticum transformation protocol and the methods for selecting for transformants are described. Methods for determining strain phenotypes are also presented.

Keywords: Metabolic engineering; Strain phenotypes; Thermoanaerobacterium saccharolyticum.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Proteins / metabolism
Enzyme Assays
Fermentation
Gene Deletion
Gene Expression Regulation, Bacterial
Genetic Engineering
Metabolic Engineering / methods*
Phenotype
Promoter Regions, Genetic / genetics
RNA, Ribosomal, 16S / genetics
Thermoanaerobacterium / genetics
Thermoanaerobacterium / metabolism*
Transformation, Genetic

Substances

Bacterial Proteins
RNA, Ribosomal, 16S

Supplementary concepts

Thermoanaerobacterium saccharolyticum