Complex assembly, crystallization and preliminary X-ray crystallographic analysis of duck MHC class I complexed with a TUMV viral peptide

Zixin Liu; Xiaoli Xie; Zhuolin Li; Lin Zhang; Nianzhi Zhang

doi:10.1016/j.rvsc.2020.07.009

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of duck MHC class I complexed with a TUMV viral peptide

Res Vet Sci. 2020 Oct:132:312-317. doi: 10.1016/j.rvsc.2020.07.009. Epub 2020 Jul 18.

Authors

Zixin Liu¹, Xiaoli Xie¹, Zhuolin Li¹, Lin Zhang², Nianzhi Zhang³

Affiliations

¹ Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
² Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, China.
³ Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, China. Electronic address: [email protected].

PMID: 32721646
DOI: 10.1016/j.rvsc.2020.07.009

Abstract

The CTL immune response mediated by MHC I plays an important role in duck anti-TMUV infection. This study reports the expression, purification and crystallization of a complex of duck MHC class I molecules Anpl-UAA*SD, duck β2-microglobulin (Anpl-β2m) and the polypeptide LRKRQLTVL (LRK9) derived from Tembusu virus (TMUV) NS3. The crystal diffraction resolution is 1.50 Å and belongs to the P6₂ space group, and the unit cell parameters are a = 82.468, b = 82.468, c = 112.507. The Matthew's constant is calculated to be 2.32 Å³ Da ^-1, and an asymmetric unit contains a complex molecule with a solvent content of 47%. The research lays the foundation for the structure of immune molecules about duck anti-TMUV research.

Keywords: Complex assembly; Crystallization; Duck; MHC class I; Peptide; TMUV.

MeSH terms

Animals
Crystallization / veterinary
Crystallography, X-Ray / veterinary
Ducks*
Flavivirus / metabolism*
Histocompatibility Antigens Class I / chemistry*
Histocompatibility Antigens Class I / metabolism
Viral Proteins / chemistry*

Substances

Histocompatibility Antigens Class I
Viral Proteins

Supplementary concepts

Tembusu virus