Identification and characterisation of fosfomycin resistance in Escherichia coli urinary tract infection isolates from Australia

Shakeel Mowlaboccus; Denise Daley; Stanley Pang; Thomas Gottlieb; John Merlino; Graeme R Nimmo; Narelle George; Tony M Korman; Richard Streitberg; Jenny Robson; Georgia Peachey; Peter Collignon; Susan Bradbury; Elena Colombi; Joshua P Ramsay; Benjamin A Rogers; Geoffrey W Coombs

doi:10.1016/j.ijantimicag.2020.106121

Identification and characterisation of fosfomycin resistance in Escherichia coli urinary tract infection isolates from Australia

Int J Antimicrob Agents. 2020 Oct;56(4):106121. doi: 10.1016/j.ijantimicag.2020.106121. Epub 2020 Jul 31.

Authors

Shakeel Mowlaboccus¹, Denise Daley², Stanley Pang³, Thomas Gottlieb⁴, John Merlino⁴, Graeme R Nimmo⁵, Narelle George⁵, Tony M Korman⁶, Richard Streitberg⁷, Jenny Robson⁸, Georgia Peachey⁴, Peter Collignon⁹, Susan Bradbury⁹, Elena Colombi¹⁰, Joshua P Ramsay¹⁰, Benjamin A Rogers¹¹, Geoffrey W Coombs³

Affiliations

¹ Murdoch University, 90 South Street, Murdoch, WA 6150, Australia. Electronic address: [email protected].
² Fiona Stanley Hospital, PathWest Laboratory Medicine WA, Murdoch, WA, Australia.
³ Murdoch University, 90 South Street, Murdoch, WA 6150, Australia; Fiona Stanley Hospital, PathWest Laboratory Medicine WA, Murdoch, WA, Australia.
⁴ Concord Hospital, Concord, NSW, Australia.
⁵ Pathology Queensland Central Laboratory, Herston, QLD, Australia.
⁶ Monash University, Clayton, VIC, Australia; Monash Pathology, Monash Health, Clayton, VIC, Australia.
⁷ Monash Pathology, Monash Health, Clayton, VIC, Australia.
⁸ Sullivan Nicolaides Pathology, QLD, Australia.
⁹ The Canberra Hospital, Garran, ACT, Australia.
¹⁰ Curtin University, Perth, WA, Australia.
¹¹ Monash University, Melbourne, VIC, Australia.

PMID: 32739475
DOI: 10.1016/j.ijantimicag.2020.106121

Abstract

Of 1033 Escherichia coli urinary tract infection isolates collected from females >12 years of age in Australia in 2019, only 2 isolates were resistant to fosfomycin with a minimum inhibitory concentration (MIC) of >256 mg/L. Despite having different multilocus sequence types, the two isolates harboured an identical plasmid-encoded fosA4 gene. The fosA4 gene has previously been identified in a single clinical E. coli isolate cultured in Japan in 2014. Each fosfomycin-resistant isolate harboured two conjugative plasmids that possessed an array of genes conferring resistance to aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides and/or trimethoprim.

Keywords: Escherichia coli; Fosfomycin resistance; Urinary tract infection; fosA4.

MeSH terms

Anti-Bacterial Agents / therapeutic use*
Australia
Child
Cross-Sectional Studies
Drug Resistance, Multiple, Bacterial / genetics
Escherichia coli / drug effects*
Escherichia coli / genetics
Escherichia coli / isolation & purification
Escherichia coli Infections / drug therapy*
Female
Fosfomycin / therapeutic use*
Genome, Bacterial
Humans
Microbial Sensitivity Tests
Plasmids / genetics
Urinary Tract Infections / drug therapy*
Urinary Tract Infections / microbiology
Whole Genome Sequencing

Substances

Anti-Bacterial Agents
Fosfomycin