Primary cilia biogenesis and associated retinal ciliopathies

Semin Cell Dev Biol. 2021 Feb:110:70-88. doi: 10.1016/j.semcdb.2020.07.013. Epub 2020 Jul 31.

Abstract

The primary cilium is a ubiquitous microtubule-based organelle that senses external environment and modulates diverse signaling pathways in different cell types and tissues. The cilium originates from the mother centriole through a complex set of cellular events requiring hundreds of distinct components. Aberrant ciliogenesis or ciliary transport leads to a broad spectrum of clinical entities with overlapping yet highly variable phenotypes, collectively called ciliopathies, which include sensory defects and syndromic disorders with multi-organ pathologies. For efficient light detection, photoreceptors in the retina elaborate a modified cilium known as the outer segment, which is packed with membranous discs enriched for components of the phototransduction machinery. Retinopathy phenotype involves dysfunction and/or degeneration of the light sensing photoreceptors and is highly penetrant in ciliopathies. This review will discuss primary cilia biogenesis and ciliopathies, with a focus on the retina, and the role of CP110-CEP290-CC2D2A network. We will also explore how recent technologies can advance our understanding of cilia biology and discuss new paradigms for developing potential therapies of retinal ciliopathies.

Keywords: CEP290; Ciliogenesis; Intracellular transport; Photoreceptor; Retinal degeneration; Sensory cilia.

Publication types

  • Research Support, N.I.H., Intramural
  • Review

MeSH terms

  • Animals
  • Antigens, Neoplasm / genetics*
  • Antigens, Neoplasm / metabolism
  • Biological Transport
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Centrioles / metabolism
  • Centrioles / ultrastructure
  • Cilia / metabolism*
  • Cilia / ultrastructure
  • Ciliopathies / genetics*
  • Ciliopathies / metabolism
  • Ciliopathies / pathology
  • Cytoskeletal Proteins / genetics*
  • Cytoskeletal Proteins / metabolism
  • Disease Models, Animal
  • Gene Expression Regulation
  • Humans
  • Light Signal Transduction
  • Mice
  • Microtubule-Associated Proteins / genetics*
  • Microtubule-Associated Proteins / metabolism
  • Microtubules / metabolism
  • Microtubules / ultrastructure
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Photoreceptor Cells, Vertebrate / metabolism
  • Photoreceptor Cells, Vertebrate / pathology
  • Retinal Degeneration / genetics*
  • Retinal Degeneration / metabolism
  • Retinal Degeneration / pathology
  • Retinitis Pigmentosa / genetics*
  • Retinitis Pigmentosa / metabolism
  • Retinitis Pigmentosa / pathology

Substances

  • Antigens, Neoplasm
  • CC2D2A protein, human
  • CCP110 protein, human
  • Cell Cycle Proteins
  • Cep290 protein, human
  • Cytoskeletal Proteins
  • Microtubule-Associated Proteins
  • Phosphoproteins