Cloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli

Int Arch Allergy Appl Immunol. 1988;85(1):127-9. doi: 10.1159/000234488.

Abstract

A cDNA clone coding for the major house dust mite allergen Der p 1 was isolated from a lambda gt 11 library. Its sequence correlates with known amino acid sequences of Der p 1 and it produces a fusion protein which reacts with rabbit anti-Der p 1 antiserum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / genetics
  • Allergens / isolation & purification*
  • Amino Acid Sequence
  • Animals
  • Antigens, Dermatophagoides
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Mites / genetics*
  • Mites / immunology
  • Molecular Sequence Data

Substances

  • Allergens
  • Antigens, Dermatophagoides