Implantable zoledronate-PLGA microcapsules ameliorate alveolar bone loss, gingival inflammation and oxidative stress in an experimental periodontitis rat model

J Biomater Appl. 2021 Jan;35(6):569-578. doi: 10.1177/0885328220944683. Epub 2020 Aug 9.

Abstract

The effect of implantable Zoledronate-PLGA microcapsules (PLGA-ZOL) in periodontitis remains unclear. In this study, we aimed to explore the potential role of PLGA-ZOL in protecting periodontitis and elucidate the underlying mechanism. A rat model of periodontitis was established by ligation the mandibular first molars, then PLGA-ZOL was implanted. The healing volume was scanned by cone-beam computed tomography. Cytokine levels in the gingival tissues were determined by ELISA and RT-PCR. Oxidative stress was indicated by detecting superoxide dismutase concentration and catalase activity. After periodontitis model was successfully established in rats, PLGA-ZOL treatment significantly attenuated alveolar bone loss, as indicated by the increased total healing volume, bone volume/tissue volume and osteoprotegerin level, as well as decreased sRANKL level. PLGA-ZOL treatment also suppressed the inflammatory activities by inhibiting pro-inflammatory cytokine production (TNF-α, IL-1β) but increasing anti-inflammatory cytokine secretion (IL-10). Furthermore, PLGA-ZOL was found to ameliorate oxidative stress in gingival tissues. In conclusion, PLGA-ZOL microcapsules ameliorate alveolar bone loss, gingival inflammation and oxidative stress in an experimental rat model of periodontitis.

Keywords: PLGA-ZOL; alveolar bone loss; inflammation; oxidative stress; periodontitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alveolar Bone Loss / drug therapy*
  • Animals
  • Antioxidants / chemistry*
  • Antioxidants / pharmacology
  • Capsules / chemistry*
  • Catalase / metabolism
  • Cytokines / metabolism
  • Disease Models, Animal
  • Gingiva / metabolism
  • Humans
  • Inflammation / drug therapy*
  • Osteoprotegerin / metabolism
  • Oxidative Stress / drug effects
  • Polylactic Acid-Polyglycolic Acid Copolymer / chemistry*
  • Prosthesis Implantation
  • Rats
  • Superoxide Dismutase / metabolism
  • Zoledronic Acid / chemistry*
  • Zoledronic Acid / pharmacology

Substances

  • Antioxidants
  • Capsules
  • Cytokines
  • Osteoprotegerin
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Zoledronic Acid
  • Catalase
  • Superoxide Dismutase