JBPOS0101 regulates amyloid beta, tau, and glial cells in an Alzheimer's disease model

PLoS One. 2020 Aug 13;15(8):e0237153. doi: 10.1371/journal.pone.0237153. eCollection 2020.

Abstract

Alzheimer's disease (AD) is the most prevalent neurodegenerative disease characterized by cognitive dysfunction and memory loss as the main symptoms. The deposition of amyloid beta (Aβ) and tau hyperphosphorylation are hallmarks of AD and are major therapeutic targets. However, the exact etiology has not yet been fully elucidated; thus, no drug that cures the disease has been approved. JBPOS0101 is a phenyl carbamate compound that has been tested as a drug for epileptic diseases. In our previous study, we showed that JBPOS0101 attenuated the accumulation of Aβ as well as the deficits in learning and memory in the 5xFAD mouse model. Here, we tested the dose effect (70 or 35 mg/kg) of JBPOS0101 on the memory defect and pathological markers and further investigated the underlying mechanisms in 5xFAD mice. In the behavior tests, JBPOS0101 treatment ameliorated deficits in learning and memory. Moreover, JBPOS0101 attenuated Aβ accumulation and tau phosphorylation. The elevated phosphorylation levels of the active GSK3β form (GSK3β-y216) in 5xFAD, which are responsible for tau phosphorylation, decreased in the JBPOS0101-treated groups. Furthermore, the elevation of reactive astrocytes and microglia in 5xFAD mice was attenuated in JBPOS0101-treated groups. These data suggest that JBPOS0101 may be a new drug candidate to lessen amyloid- and tau-related pathology by regulating glial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy*
  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Female
  • Glycogen Synthase Kinase 3 beta / metabolism
  • Memory
  • Mice
  • Microglia / drug effects*
  • Microglia / metabolism
  • Neuroprotective Agents / pharmacology*
  • Neuroprotective Agents / therapeutic use
  • tau Proteins / metabolism*

Substances

  • Amyloid beta-Peptides
  • Neuroprotective Agents
  • tau Proteins
  • Glycogen Synthase Kinase 3 beta

Grants and funding

This research was funded by Bio-Pharm Solutions Co. Ltd, Korea and supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT, 2019R1A2C1004575, WSC). The funder provided support in the form of salaries for authors JKJ and YMC, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.