Generation of induced pluripotent stem cell lines ICGi021-A and ICGi022-A from peripheral blood mononuclear cells of two healthy individuals from Siberian population

A A Malakhova; E V Grigor'eva; S V Pavlova; T B Malankhanova; K R Valetdinova; Y V Vyatkin; E A Khabarova; J A Rzaev; S M Zakian; S P Medvedev

doi:10.1016/j.scr.2020.101952

Generation of induced pluripotent stem cell lines ICGi021-A and ICGi022-A from peripheral blood mononuclear cells of two healthy individuals from Siberian population

Stem Cell Res. 2020 Oct:48:101952. doi: 10.1016/j.scr.2020.101952. Epub 2020 Aug 8.

Authors

Affiliations

¹ Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia. Electronic address: [email protected].
² Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
³ Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
⁴ Novosibirsk State University, Novosibirsk, Russia; AcademGene LLC, Novosibirsk, Russia.
⁵ FSBI Federal Neurosurgical Center, Novosibirsk, Russia.

PMID: 32805537
DOI: 10.1016/j.scr.2020.101952

Abstract

ICGi021-A and ICGi022-A iPSC lines were obtained by reprogramming PBMCs of two healthy women of the Siberian population using episomal non-integrating vectors expressing Yamanaka factors. iPSC lines expressed pluripotency markers, had a normal karyotype and demonstrated the ability to differentiate into derivatives of the three germ layers. Clinical exome sequencing data of the original biosamples of the donors are available in the NCBI SRA database. The generated cell lines are useful as "healthy" control in biomedical studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Line
Cellular Reprogramming
Female
Germ Layers
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear*
Siberia