Current approaches for detection of hydrogen sulfide and persulfidation in biological systems

The past decades have witnessed hydrogen sulfide (H₂S) serving as gaseous signaling molecule participating in diverse cellular and physiological processes in biological systems. Recently, a considerable number of studies highlight the signaling role of this redox-regulating molecule occurs via persulfidation, which is a post-translation modification of protein cysteine residues by covalent addition of thiol group form persulfide. However, our current understanding on detection of H₂S and persulfidation in biological systems still lags behind. This review aims to summarize current approaches for measuring H₂S and persulfidated levels in biological systems. Meanwhile, potential interference may exist in plant research has been proposed and discussed.