Abstract
The extent of DNA ethylation and the influence of excision repair on ethyl methanesulfonate (EMS) mutagenesis of Salmonella typhimurium were examined. The relationship between the dose to DNA and the exposure concentration of EMS was linear. EMS induction of his+ revertants followed exponential kinetics and did not parallel the increase in total DNA ethylation. Mutant induction was influenced by the cells' nucleotide excision repair ability. Although mutagenized to a larger extent than the wild-type (uvr+) strain at high doses, the uvrB strain was more resistant to the mutagenic effect of low doses of EMS.
MeSH terms
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Alkylation
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology*
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DNA Damage
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DNA Repair
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DNA, Bacterial / drug effects*
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DNA, Bacterial / metabolism
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Dose-Response Relationship, Drug
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Drug Resistance, Microbial
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Endodeoxyribonucleases / genetics
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Endodeoxyribonucleases / physiology*
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Escherichia coli Proteins*
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Ethyl Methanesulfonate / metabolism
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Ethyl Methanesulfonate / pharmacology*
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Mutation
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Salmonella typhimurium / drug effects*
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Salmonella typhimurium / genetics
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Salmonella typhimurium / metabolism
Substances
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Bacterial Proteins
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DNA, Bacterial
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Escherichia coli Proteins
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Ethyl Methanesulfonate
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Endodeoxyribonucleases
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endodeoxyribonuclease uvrABC