A cautionary tale of pyridoxine toxicity in cystathionine beta-synthase deficiency detected by two-tier newborn screening highlights the need for clear pyridoxine dosing guidelines

Am J Med Genet A. 2020 Nov;182(11):2704-2708. doi: 10.1002/ajmg.a.61815. Epub 2020 Aug 20.

Abstract

Classic homocystinuria is due to deficiency of cystathionine beta-synthase (CBS), a pyridoxine-dependent enzyme that, depending on the molecular variants, may be co-factor responsive. Elevated methionine is often used as the primary analyte to detect CBS deficiency (CBSD) on newborn screening (NBS), but is limited by increased detection of other biochemical disorders with less clear clinical significance such as methionine aminotransferase (MAT) I/III heterozygotes. Our state has implemented a two-tier NBS algorithm for CBSD that successfully reduced the number of MATI/III heterozygotes, yet effectively detected a mild, co-factor responsive form of CBSD. After initial diagnosis, newborns with CBSD often undergo a pyridoxine challenge with high-dose pyridoxine to determine responsiveness. Here we describe our NBS-identified patient with a mild form of pyridoxine responsive CBSD who developed respiratory failure and rhabdomyolysis consistent with pyridoxine toxicity during a pyridoxine challenge. This case highlights the need for weight-based dosing and duration recommendations for pyridoxine challenge in neonates.

Keywords: homocystinuria; hypermethioninemia; pyridoxine responsive.

Publication types

  • Case Reports

MeSH terms

  • Cystathionine beta-Synthase / deficiency*
  • Cystathionine beta-Synthase / genetics*
  • Dose-Response Relationship, Drug
  • Female
  • Homocystinuria / drug therapy*
  • Homocystinuria / genetics
  • Homocystinuria / pathology
  • Humans
  • Infant, Newborn
  • Neonatal Screening / methods*
  • Prognosis
  • Pyridoxine / administration & dosage
  • Pyridoxine / adverse effects*
  • Respiratory Insufficiency / chemically induced
  • Respiratory Insufficiency / pathology*
  • Rhabdomyolysis / chemically induced
  • Rhabdomyolysis / pathology*
  • Vitamin B Complex / administration & dosage
  • Vitamin B Complex / adverse effects

Substances

  • Vitamin B Complex
  • Cystathionine beta-Synthase
  • Pyridoxine