Antibacterial activity and rice-induced resistance, mediated by C₁₅surfactin A, in controlling rice disease caused by Xanthomonas oryzae pv. oryzae

Xanthomonas oryzae pv. oryzae (Xoo) is an important pathogen in rice. C₁₅surfactin A, produced by Bacillus velezensis HN-2, displayed antibacterial activity against Xoo and effectively inhibited its infection of rice. The median inhibitory concentration of C₁₅surfactin A was 9.27 μg/mL. Scanning electron and transmission electron microscopy examination showed that C₁₅surfactin A caused significant damage to the cell wall structure of Xoo cells. On the other hand, dramatic increases in the activity of phenylalanine ammonia-lyase (Pal) and H₂O₂ content were observed in rice leaves inoculated with Xoo from 0 h to 72 h. Quantitative PCR assays indicated that C₁₅surfactin A exposure upregulated the expression of the genes Pr1a, CatA, and Pal. The results showed that C₁₅surfactin A could inhibit the growth of Xoo and effectively induce rice resistance to Xoo by triggering a hypersensitive reaction (HR) via mediation of the activities of antioxidant-related enzymes. Taken together, C₁₅surfactin A has strong antibiotic activity against Xoo and effectively induces rice resistance to Xoo. These results highlight the potential of C₁₅surfactin A as a biocontrol agent against Xoo in rice.