Mathematical modelling of the mechano-electric coupling in the human cardiomyocyte electrically connected with fibroblasts

Prog Biophys Mol Biol. 2021 Jan:159:46-57. doi: 10.1016/j.pbiomolbio.2020.08.003. Epub 2020 Aug 23.

Abstract

Cardiac fibroblasts are interspersed within mammalian cardiac tissue. Fibroblasts are mechanically passive; however, they may communicate electrically with cardiomyocytes via gap junctions and thus affect the electrical and mechanical activity of myocytes. Several in-silico studies at both cellular (0D) and ventricular (3D) levels analysed the effects of fibroblasts on the myocardial electrical function. However, none of them addressed possible effects of fibroblast-myocyte electrical coupling to cardiomyocyte mechanical activity. In this paper, we propose a mathematical model for studying both electrical and mechanical responses of the human cardiomyocyte to its electrotonic interaction with cardiac fibroblasts. Our simulations have revealed that electrotonic interaction with fibroblasts affects not only the mechanical activity of the cardiomyocyte, comprising either moderate or significant reduction of contractility, but also the mechano-calcium and mechano-electric feedback loops, and all these effects are enhanced as the number of coupled fibroblasts is increased. Obtained results suggest that moderate values of the myocyte-fibroblast gap junction conductance (less than 1 nS) can be attributed to physiological conditions, contrasting to the higher values (2 nS and higher) proper rather for pathological situations (e.g. for infarct and/or border zones), since all mechanical indexes falls down dramatically in the case of such high conductance.

Keywords: Cardiac electromechanics; Cell modelling; Fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomechanical Phenomena / physiology*
  • Calcium / metabolism
  • Cell Communication / physiology
  • Electrophysiology
  • Fibroblasts / metabolism*
  • Gap Junctions / metabolism*
  • Ion Channels / metabolism
  • Models, Biological*
  • Models, Theoretical
  • Myocardium / cytology
  • Myocytes, Cardiac / metabolism*
  • Potassium / metabolism
  • Ryanodine Receptor Calcium Release Channel / metabolism
  • Sarcoplasmic Reticulum / metabolism
  • Sodium / metabolism

Substances

  • Ion Channels
  • Ryanodine Receptor Calcium Release Channel
  • Sodium
  • Potassium
  • Calcium