Impacts of non-Saccharomyces species and aeration on sequential inoculation with Saccharomyces cerevisiae to produce lower alcohol Merlot wines from Washington state

J Sci Food Agric. 2021 Mar 15;101(4):1715-1719. doi: 10.1002/jsfa.10769. Epub 2020 Sep 16.

Abstract

Background: Species of non-Saccharomyces yeasts isolated from Washington vineyards were evaluated for their abilities to reduce alcohol contents of wines. As many of these yeasts benefit from some oxygen, the effect of limited aeration was also studied.

Results: Although fermentations of a high sugar Merlot grape must (310 g L-1 ) did not reach dryness, inoculation of Metschnikowia chrysoperlae, Mt. pulcherrima, Meyerozyma guillermondii, Pichia kluyveri, or P. membranifaciens yielded in wines with lower amounts of ethanol without excessive levels of acetic acid. Aeration frequently resulted in wines with less ethanol but with more acetic acid compared to non-aerated fermentations. Inoculation of Mt. pulcherrima or My. guilliermondii into another Merlot grape must that contained a lower initial amount of fermentable sugar (266 g L-1 ) resulted in dry wines that contained less alcohol.

Conclusions: Inoculation of My. guilliermondii or Mt. pulcherrima before primary alcoholic fermentation resulted in wines with reduced alcohol contents without excessive acetic acid production. © 2020 Society of Chemical Industry.

Keywords: ethanol; non-Saccharomyces yeast; sequential fermentation; wine; yeast metabolism.

Publication types

  • Evaluation Study

MeSH terms

  • Acetic Acid / analysis
  • Acetic Acid / metabolism
  • Ethanol / analysis
  • Ethanol / metabolism*
  • Fermentation
  • Food Microbiology / methods*
  • Fruit / chemistry
  • Fruit / metabolism
  • Fruit / microbiology
  • Metschnikowia / metabolism*
  • Pichia / metabolism*
  • Saccharomyces cerevisiae / metabolism*
  • Vitis / chemistry
  • Vitis / metabolism
  • Vitis / microbiology*
  • Washington
  • Wine / analysis*
  • Wine / microbiology

Substances

  • Ethanol
  • Acetic Acid

Supplementary concepts

  • Pichia kluyveri