Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins

Hongwei Sun; Shengyao Zhi; Guifang Wu; Guanglan Wu; Tianqi Cao; Hu Hao; Zhou Songyang; Puping Liang; Junjiu Huang

doi:10.1016/j.jgg.2020.05.005

Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins

J Genet Genomics. 2020 Jun 20;47(6):337-340. doi: 10.1016/j.jgg.2020.05.005. Epub 2020 May 20.

Authors

Hongwei Sun¹, Shengyao Zhi¹, Guifang Wu¹, Guanglan Wu¹, Tianqi Cao¹, Hu Hao², Zhou Songyang³, Puping Liang⁴, Junjiu Huang⁵

Affiliations

¹ MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
² Department of Paediatrics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China.
³ MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences, and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China; Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA. Electronic address: [email protected].
⁴ MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences, and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address: [email protected].
⁵ MOE Key Laboratory of Gene Function and Regulation and Guangzhou Key Laboratory of Healthy Aging Research, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences, and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China. Electronic address: [email protected].

PMID: 32888879
DOI: 10.1016/j.jgg.2020.05.005

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Adenine / metabolism
Animals
CRISPR-Cas Systems / genetics*
Cost-Benefit Analysis*
Electroporation / economics
Gene Editing / economics
Humans
Mice
RNA, Guide, CRISPR-Cas Systems
Ribonucleoproteins / genetics*

Substances

Ribonucleoproteins
Adenine