Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells

Int J Mol Sci. 2020 Sep 4;21(18):6455. doi: 10.3390/ijms21186455.

Abstract

Mesenchymal stem cells (MSCs) are commonly isolated from bone marrow and adipose tissue. Depending on the tissue of origin, MSCs have different characteristics and physiological effects. In various cancer studies, MSCs have been found to have either tumor-promoting or tumor-inhibiting action. This study investigated the effect of adipose tissue-MSCs (AT-MSCs) and bone marrow-MSCs (BM-MSCs) on global long interspersed nuclear element-1 (LINE-1) methylation, the expression level of microenvironment remodeling genes and cell proliferation, migration and invasion of oral tongue squamous cell carcinoma (OTSCC). Additionally, we studied the effect of human tongue squamous carcinoma (HSC-3)-conditioned media on LINE-1 methylation and the expression of microenvironment remodeling genes in AT-MSCs and BM-MSCs. Conditioned media from HSC-3 or MSCs did not affect LINE-1 methylation level in either cancer cells or MSCs, respectively. In HSC-3 cells, no effect of MSCs-conditioned media was detected on the expression of ICAM1, ITGA3 or MMP1. On the other hand, HSC-3-conditioned media upregulated ICAM1 and MMP1 expression in both types of MSCs. Co-cultures of AT-MSCs with HSC-3 did not induce proliferation, migration or invasion of the cancer cells. In conclusion, AT-MSCs, unlike BM-MSCs, seem not to participate in oral cancer progression.

Keywords: adipose tissue; bone marrow; invasion; mesenchymal stem cells; migration; oral squamous cell carcinoma.

MeSH terms

  • Adipose Tissue / metabolism
  • Adipose Tissue / pathology
  • Bone Marrow / metabolism
  • Bone Marrow / pathology
  • Bone Marrow Cells / cytology
  • Carcinoma, Squamous Cell / pathology
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Coculture Techniques
  • Culture Media, Conditioned / pharmacology
  • DNA Methylation / drug effects
  • Head and Neck Neoplasms / pathology
  • Humans
  • Long Interspersed Nucleotide Elements / drug effects
  • Long Interspersed Nucleotide Elements / genetics
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Mesenchymal Stem Cells / physiology
  • Mouth Neoplasms / pathology
  • Neoplasm Invasiveness / physiopathology
  • Squamous Cell Carcinoma of Head and Neck / metabolism*
  • Squamous Cell Carcinoma of Head and Neck / pathology*
  • Tongue Neoplasms / pathology
  • Tumor Microenvironment / drug effects

Substances

  • Culture Media, Conditioned