Comparison of four commercial SARS-CoV-2 IgG immuno-assays in RT-PCR negative patients with suspect CT findings

Infection. 2021 Feb;49(1):145-148. doi: 10.1007/s15010-020-01523-3. Epub 2020 Sep 10.

Abstract

A subset of patients with Covid-19 presents with negative RT-PCR screening but suspect CT findings. Using four commercially available anti-SARS-CoV-2 IgG immuno-assays, we found this subset constituted 9.2% of all consecutively admitted outpatients with Covid-19 in our hospital. Clinical specificity for Covid-19 of some N protein-based immuno-assays was suboptimal, as positive results were observed in control patients with recent common human coronavirus, influenza B and adenovirus infections.

Keywords: Covid-19; ELISA; SARS-CoV-2; Serology.

Publication types

  • Comparative Study

MeSH terms

  • Adenovirus Infections, Human / diagnosis
  • Adenovirus Infections, Human / immunology
  • Adenovirus Infections, Human / virology
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • COVID-19 / diagnosis*
  • COVID-19 / immunology
  • COVID-19 / virology
  • COVID-19 Testing / standards*
  • Child
  • Child, Preschool
  • Coronavirus Infections / diagnosis
  • Coronavirus Infections / immunology
  • Coronavirus Infections / virology
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / statistics & numerical data*
  • False Positive Reactions
  • Female
  • Humans
  • Infant
  • Influenza, Human / diagnosis
  • Influenza, Human / immunology
  • Influenza, Human / virology
  • Male
  • Middle Aged
  • Reverse Transcriptase Polymerase Chain Reaction
  • SARS-CoV-2 / genetics
  • SARS-CoV-2 / immunology*
  • Sensitivity and Specificity
  • Tomography, X-Ray Computed