Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

Sci Rep. 2020 Sep 17;10(1):15250. doi: 10.1038/s41598-020-72132-1.

Abstract

The use of faecal microbial markers as non-invasive biomarkers for colorectal cancer (CRC) has been suggested, but not fully elucidated. Here, we have evaluated the importance of Parvimonas micra as a potential non-invasive faecal biomarker in CRC and its relation to other microbial biomarkers. The levels of P. micra, F. nucleatum and clbA + bacteria were quantified using qPCR in faecal samples from a population-based cohort of patients undergoing colonoscopy due to symptoms from the large bowel. The study included 38 CRC patients, 128 patients with dysplasia and 63 controls. The results were validated in a second consecutive CRC cohort including faecal samples from 238 CRC patients and 94 controls. We found significantly higher levels of P. micra in faecal samples from CRC patients compared to controls. A test for P. micra could detect CRC with a specificity of 87.3% and a sensitivity of 60.5%. In addition, we found that combining P. micra with other microbial markers, could further enhance test sensitivity. Our findings support the potential use of P. micra as a non-invasive biomarker for CRC. Together with other microbial faecal markers, P. micra may identify patients with "high risk" microbial patterns, indicating increased risk and incidence of cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Bacterial Load
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / isolation & purification
  • Case-Control Studies
  • Cohort Studies
  • Colonoscopy
  • Colorectal Neoplasms / diagnosis*
  • Colorectal Neoplasms / microbiology*
  • Early Detection of Cancer / methods
  • Feces / microbiology
  • Female
  • Firmicutes / genetics
  • Firmicutes / isolation & purification*
  • Fusobacterium nucleatum / genetics
  • Fusobacterium nucleatum / isolation & purification
  • Gastrointestinal Microbiome* / genetics
  • Humans
  • Male
  • Middle Aged
  • Risk Factors

Substances

  • Biomarkers, Tumor

Supplementary concepts

  • Parvimonas micra