In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae

J Bacteriol. 1987 Jun;169(6):2876-80. doi: 10.1128/jb.169.6.2876-2880.1987.

Abstract

In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by covalent modification (A.J. Ninfa and B. Magasanik, Proc. Natl. Acad. Sci. USA 83:5909, 1986). Two DNA-binding sites for NTRC lie approximately 150 base pairs upstream of the nifLA promoter.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • DNA-Binding Proteins / genetics
  • Gene Expression Regulation
  • Genes, Bacterial
  • In Vitro Techniques
  • Klebsiella pneumoniae / genetics*
  • Nitrogen Fixation*
  • Operon
  • PII Nitrogen Regulatory Proteins
  • Promoter Regions, Genetic*
  • Sigma Factor / genetics
  • Trans-Activators*
  • Transcription Factors / genetics
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • PII Nitrogen Regulatory Proteins
  • Sigma Factor
  • Trans-Activators
  • Transcription Factors