Abstract
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by covalent modification (A.J. Ninfa and B. Magasanik, Proc. Natl. Acad. Sci. USA 83:5909, 1986). Two DNA-binding sites for NTRC lie approximately 150 base pairs upstream of the nifLA promoter.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins*
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Base Sequence
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Cloning, Molecular
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DNA, Bacterial / genetics
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DNA-Binding Proteins / genetics
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Gene Expression Regulation
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Genes, Bacterial
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In Vitro Techniques
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Klebsiella pneumoniae / genetics*
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Nitrogen Fixation*
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Operon
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PII Nitrogen Regulatory Proteins
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Promoter Regions, Genetic*
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Sigma Factor / genetics
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Trans-Activators*
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Transcription Factors / genetics
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Transcription, Genetic
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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PII Nitrogen Regulatory Proteins
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Sigma Factor
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Trans-Activators
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Transcription Factors