Silencing circular RNA hsa_circ_009755 promotes growth and metastasis of oral squamous cell carcinoma

Biru Zhang; Zihong Wang; Yuehong Shen; Hongyu Yang

doi:10.1016/j.ygeno.2020.09.035

Silencing circular RNA hsa_circ_009755 promotes growth and metastasis of oral squamous cell carcinoma

Genomics. 2020 Nov;112(6):5275-5281. doi: 10.1016/j.ygeno.2020.09.035. Epub 2020 Sep 18.

Authors

Biru Zhang¹, Zihong Wang², Yuehong Shen³, Hongyu Yang⁴

Affiliations

¹ Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen 518036, China; Peking University Shenzhen Hospital Clinical College, Anhui Medical University, Hefei 230032, China; Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, China.
² Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen 518036, China; Shenzhen Baoan Maternal and Child Health Hospital, Shenzhen 518000, China; Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, China.
³ Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen 518036, China; Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, China. Electronic address: [email protected].
⁴ Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen 518036, China; Peking University Shenzhen Hospital Clinical College, Anhui Medical University, Hefei 230032, China; Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, China. Electronic address: [email protected].

PMID: 32956844
DOI: 10.1016/j.ygeno.2020.09.035

Abstract

Objective: Oral squamous cell carcinoma (OSCC) is a common and rampant malignancy of the head and neck. However, its pathogenesis remains unclear. In this study, we have investigated the effects of circular RNA hsa_circ_009755 on proliferation, migration, invasion, and apoptosis in OSCC cells in vitro.

Method: Eight pairs of OSCC and normal adjacent tissues were selected to detect the differential expression of circRNAs by high-throughput sequencing. circRNA hsa_circ_009755 expression was determined by quantitative real-time polymerase chain reaction in OSCC tissues and cell lines. CCK-8, wound healing, Transwell, and flow cytometry assays were used to determine OSCC cell proliferation, migration, invasion, and apoptosis, respectively.

Result: The expression of hsa_circ_009755 was low in OSCC tissues and three OSCC cell lines. Silencing hsa_circ_009755 significantly enhanced the proliferation, migration, and invasion and suppressed the apoptosis of OSCC cells.

Conclusion: Therefore, hsa_circ_009755 may be important in the tumorigenesis of OSCC.

Keywords: Circular RNA; Growth; Metastasis; Oral squamous cell carcinoma; hsa_circ_009755.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis
Cell Movement
Cell Proliferation
Gene Silencing
Humans
MicroRNAs / metabolism
Mouth Neoplasms / genetics*
Mouth Neoplasms / metabolism
Mouth Neoplasms / pathology*
Neoplasm Invasiveness
RNA, Circular / genetics
RNA, Circular / metabolism
RNA, Circular / physiology*
Squamous Cell Carcinoma of Head and Neck / genetics*
Squamous Cell Carcinoma of Head and Neck / metabolism
Squamous Cell Carcinoma of Head and Neck / pathology*
Squamous Cell Carcinoma of Head and Neck / secondary

Substances

MicroRNAs
RNA, Circular