Generation of two human induced pluripotent stem cell lines derived from myoblasts (MDCi014-A) and from peripheral blood mononuclear cells (MDCi014-B) from the same donor

Eric Metzler; Narasimha Telugu; Sebastian Diecke; Simone Spuler; Helena Escobar

doi:10.1016/j.scr.2020.101998

Generation of two human induced pluripotent stem cell lines derived from myoblasts (MDCi014-A) and from peripheral blood mononuclear cells (MDCi014-B) from the same donor

Stem Cell Res. 2020 Oct:48:101998. doi: 10.1016/j.scr.2020.101998. Epub 2020 Sep 17.

Authors

Eric Metzler¹, Narasimha Telugu², Sebastian Diecke³, Simone Spuler⁴, Helena Escobar¹

Affiliations

¹ Muscle Research Unit, Experimental and Clinical Research Center, A Joint Cooperation of Charité, Universitätsmedizin Berlin and the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
² Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
³ Berlin Institute of Health (BIH), Berlin, Germany; DZHK (German Centre for Cardiovascular Research), partner site Berlin, Germany; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
⁴ Muscle Research Unit, Experimental and Clinical Research Center, A Joint Cooperation of Charité, Universitätsmedizin Berlin and the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Charité, Universitätsmedizin Berlin, Germany.

PMID: 32979629
DOI: 10.1016/j.scr.2020.101998

Abstract

We describe the generation and characterization of two human induced pluripotent stem cell (hiPSCs) lines reprogrammed from myoblasts and from peripheral blood mononuclear cells (PBMCs) from the same donor. The donor was free of neuromuscular disorders, male and 18 years of age. For reprogramming we used Sendai-virus delivery of the four Yamanaka factors. The pluripotent identity of the hiPSC lines was confirmed by the expression of pluripotency markers and their capacity to differentiate into all three germ layers. These hiPSCs constitute a tool to study tissue of origin specific differences in the identity of hiPSCs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Male
Myoblasts