Emergence of ST15 Klebsiella pneumoniae Clinical Isolates Producing Plasmids-Mediated RmtF and OXA-232 in China

Qingyu Shi; Renru Han; Yan Guo; Yonggui Zheng; Yang Yang; Dandan Yin; Rong Zhang; Fupin Hu

doi:10.2147/IDR.S257298

Emergence of ST15 Klebsiella pneumoniae Clinical Isolates Producing Plasmids-Mediated RmtF and OXA-232 in China

Infect Drug Resist. 2020 Sep 11:13:3125-3129. doi: 10.2147/IDR.S257298. eCollection 2020.

Authors

Qingyu Shi^{1

2}, Renru Han^{1

2}, Yan Guo^{1

2}, Yonggui Zheng^{1

2}, Yang Yang^{1

2}, Dandan Yin^{1

2}, Rong Zhang³, Fupin Hu^{1

2}

Affiliations

¹ Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
² Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, People's Republic of China.
³ Department of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang University, Hangzhou, People's Republic of China.

Abstract

Background: RmtF, as 16S rRNA methyltransferase, leads to high-level resistance to aminoglycoside and is now barely reported.

Methods and results: Three rmtF-positive Klebsiella pneumoniae isolates, belonging to the pandemic clone sequence type 15, were isolated from children and coproduced bla _OXA-232 and bla _CTX-M-15. The rmtF gene was located on an IncFIB transformable plasmid of 128,536-bp and bla _OXA-232 was on a 6141-bp ColKP3 plasmid, respectively.

Conclusion: Plasmids with rmtF found worldwide, shared relatively low similarity, and merely matched partly in their multidrug resistance region. Notably, clinical isolates coproducing rmtF and bla _OXA-232 are gradually increasing in China.

Keywords: Klebsiella pneumoniae; RmtF; ST15; aminoglycoside; blaOXA-232.

Grants and funding

This work was supported by the National Natural Science Foundation of China (grant no. 81871690, 81902101) and the National Mega-project for Innovative Drugs (2019ZX09721001-006-004). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.