Chemically modified mRNA nucleofection of primary human T cells

J Immunol Methods. 2020 Dec:487:112878. doi: 10.1016/j.jim.2020.112878. Epub 2020 Oct 6.

Abstract

Here we show that an approach of in-vitro transcribed mRNA nucleofection expands the range of transfection of primary human T cells. It represents a reproducible and time-efficient technology, and is thus an ideal tool in basic research involving highly controlled in-vitro experiments with a gene of interest aiming at identifying its biological human T cell function.

Keywords: In-vitro transcribed mRNA; NR2F6; Nucleofection; Primary human T cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Green Fluorescent Proteins / biosynthesis
  • Green Fluorescent Proteins / genetics
  • Humans
  • Jurkat Cells
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • Repressor Proteins / biosynthesis*
  • Repressor Proteins / genetics
  • T-Lymphocytes / metabolism*
  • Time Factors
  • Transcription, Genetic*
  • Transfection*

Substances

  • NR2F6 protein, human
  • RNA, Messenger
  • Repressor Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins