Plasmon-enhanced quantum dots electrochemiluminescence aptasensor for selective and sensitive detection of cardiac troponin I

Shimeles Addisu Kitte; Terefe Tafese; Chen Xu; Muhammad Saqib; Haijuan Li; Yongdong Jin

doi:10.1016/j.talanta.2020.121674

Plasmon-enhanced quantum dots electrochemiluminescence aptasensor for selective and sensitive detection of cardiac troponin I

Talanta. 2021 Jan 1:221:121674. doi: 10.1016/j.talanta.2020.121674. Epub 2020 Sep 17.

Authors

Shimeles Addisu Kitte¹, Terefe Tafese², Chen Xu², Muhammad Saqib³, Haijuan Li³, Yongdong Jin⁴

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; Department of Chemistry, College of Natural Sciences, Jimma University, P. O. Box 378, Jimma, Ethiopia.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of Science and Technology of China, Hefei, 230026, PR China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of Science and Technology of China, Hefei, 230026, PR China. Electronic address: [email protected].

PMID: 33076177
DOI: 10.1016/j.talanta.2020.121674

Abstract

The development of highly sensitive electrochemiluminescence (ECL) immunosensors by using functional nanoparticles as signal amplifiers is a solution towards sensitive determination of many low concentration disease biomarkers. Herein, a sensitive aptamer-based, sandwich-type surface plasmon enhanced electrochemiluminescence (SPEECL) immunosensor was demonstrated for the detection of cardiac troponin I (cTnI), by means of aptamer conjugated CdS QDs and AuNPs as ECL luminophores and plasmon sources, respectively, in which Tro4 aptamer was used as a capture probe for cTnI and Tro6 aptamer as a detecting probe. The signal of the developed SPEECL system showed ~ 5-fold increment as compared to that of without AuNPs. Using this ECL platform for the detection of cTnI, a linear range and the limit of detection (LOD) were found to be 1 fg/mL - 10 ng/mL and 0.75 fg/mL, respectively.

Keywords: Aptamer; Surface plasmon enhanced electrochemiluminescence; cTnI.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Electrochemical Techniques
Gold
Immunoassay
Limit of Detection
Luminescent Measurements
Metal Nanoparticles*
Quantum Dots*
Troponin I

Substances

Aptamers, Nucleotide
Troponin I
Gold