Abstract
The CR3022 antibody, selected from a group of SARS-CoV monoclonal antibodies for its ability to cross-react with SARS-CoV-2, has been examined for its ability to bind to the ectodomain of the SARS-CoV-2 spike glycoprotein. Using cryo-electron microscopy we show that antibody binding requires rearrangements in the S1 domain that result in dissociation of the spike.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / immunology*
-
Antibodies, Viral / immunology*
-
Betacoronavirus / immunology*
-
Binding Sites, Antibody / immunology*
-
COVID-19
-
Cell Line
-
Chlorocebus aethiops
-
Coronavirus Infections / virology
-
Cryoelectron Microscopy
-
Humans
-
Neutralization Tests
-
Pandemics
-
Pneumonia, Viral / virology
-
Protein Domains / immunology
-
SARS-CoV-2
-
Spike Glycoprotein, Coronavirus / immunology*
-
Vero Cells
Substances
-
Antibodies, Monoclonal
-
Antibodies, Viral
-
Spike Glycoprotein, Coronavirus
-
spike protein, SARS-CoV-2