Generation of four iPSC lines from Neurofibromatosis Type 1 patients

Kiymet Bozaoglu; Wei Shern Lee; Kristina M Haebich; Kathryn N North; Jonathan M Payne; Paul J Lockhart

doi:10.1016/j.scr.2020.102013

Generation of four iPSC lines from Neurofibromatosis Type 1 patients

Stem Cell Res. 2020 Dec:49:102013. doi: 10.1016/j.scr.2020.102013. Epub 2020 Oct 1.

Authors

Kiymet Bozaoglu¹, Wei Shern Lee², Kristina M Haebich², Kathryn N North², Jonathan M Payne³, Paul J Lockhart²

Affiliations

¹ Murdoch Children's Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia. Electronic address: [email protected].
² Murdoch Children's Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia.
³ Murdoch Children's Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia; Royal Children's Hospital, Parkville, VIC, Australia.

PMID: 33091851
DOI: 10.1016/j.scr.2020.102013

Abstract

We describe the generation and characterisation of four human induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells (PBMC) from individuals with neurofibromatosis type (NF1). PBMC reprogramming was performed using a non-integrative Sendai virus containing the reprogramming factors OCT4, SOX2, MYC and KLF4. All iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry (EPCAM, TRA-1-81, SSEA1 and CD9) and immunofluorescence (OCT4 and Nanog). Differentiation of the cells into the three embryonic germ layers was confirmed using immunofluorescence. These iPSC lines are a valuable pre-clinical resource to study the molecular mechanisms underlying NF1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells*
Kruppel-Like Factor 4
Leukocytes, Mononuclear
Neurofibromatosis 1* / genetics
Sendai virus