Establishment of three human induced pluripotent stem cell lines from a type 1 diabetic family harboring sequence variants associated with autoimmunity

Edward Po-Fan Chu; Chia-Hung Lin; Candy Hsin-Hua Cho; I-Fen Cheng; Tzu-Chien Kuo; Ruei-Ying Chen; Chia-Nan Liao; Jen-Chien Cheng; Jason Isheng Tsai; Po-Chuan Wang; Shing-Jyh Chang; Chia-Ning Shen

doi:10.1016/j.scr.2020.102029

Establishment of three human induced pluripotent stem cell lines from a type 1 diabetic family harboring sequence variants associated with autoimmunity

Stem Cell Res. 2020 Dec:49:102029. doi: 10.1016/j.scr.2020.102029. Epub 2020 Oct 8.

Authors

Affiliations

¹ Genomics Research Center, Academia Sinica, Taipei, Taiwan.
² Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang Gung Memorial Hospital, Linkou, Taiwan.
³ Genomics Research Center, Academia Sinica, Taipei, Taiwan; Departiment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan.
⁴ Departiment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan.
⁵ Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.
⁶ Division of Gastroenterology, Department of Internal Medicine, Hsinchu MacKay Memorial Hospital, Hsinchu, Taiwan.
⁷ Department of Obstetrics and Gynecology, Hsinchu MacKay Memorial Hospital, Hsinchu, Taiwan.
⁸ Genomics Research Center, Academia Sinica, Taipei, Taiwan; Departiment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan. Electronic address: [email protected].

PMID: 33096384
DOI: 10.1016/j.scr.2020.102029

Abstract

Type 1 diabetes (T1D) is characterized by the autoimmune destruction of insulin-producing β cells. Genetic studies have identified > 60 T1D risk loci that harbor genes with disease-causative alleles. However, determining the biological effects of such loci is often difficult due to limited tissue availability. Disease-specific human induced pluripotent stem cells (hiPSCs) are a valuable resource for modeling T1D pathogenesis. In particular, families with complete disease penetrance offer an opportunity to further dissect T1D risk loci. Here, we describe the generation of three hiPSC lines from a T1D family with sequence variants associated with autoimmunity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Autoimmunity / genetics
Diabetes Mellitus, Type 1* / genetics
Humans
Induced Pluripotent Stem Cells*
Insulin-Secreting Cells*