Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis

Eszter Szabó; Dóra Reé; Bálint Jezsó; Katalin Vincze; Gábor Földes; Andrea Á Molnár; János M Réthelyi; Ágota Apáti

doi:10.1016/j.scr.2020.102051

Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis

Stem Cell Res. 2020 Dec:49:102051. doi: 10.1016/j.scr.2020.102051. Epub 2020 Oct 15.

Authors

Eszter Szabó¹, Dóra Reé¹, Bálint Jezsó¹, Katalin Vincze², Gábor Földes³, Andrea Á Molnár³, János M Réthelyi⁴, Ágota Apáti⁵

Affiliations

¹ Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary.
² Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary; Molecular Psychiatry Research Group, National Brain Research Program (NAP), Hungarian Academy of Sciences and Semmelweis University, Hungary.
³ Heart and Vascular Center, Semmelweis University, Budapest, Hungary.
⁴ Molecular Psychiatry Research Group, National Brain Research Program (NAP), Hungarian Academy of Sciences and Semmelweis University, Hungary; Department of Psychiatry and Psychotherapy, Semmelweis University, Budapest, Hungary.
⁵ Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary. Electronic address: [email protected].

PMID: 33099106
DOI: 10.1016/j.scr.2020.102051

Abstract

Here we describe the generation of induced pluripotent stem cell (iPSC) lines from peripheral blood samples of identical twin sisters with type 2 diabetes mellitus (DM2). Two clonal lines from each patient (HU-DM2-A-1, HU-DM2-A-2 and HU-DM2-B-1, HU-DM2-B-2) were established via Sendai viral reprograming of peripheral blood mononuclear cells, and characterized to confirm pluripotency and genetic integrity. The established iPSC lines can help to investigate DM2 related cellular phenotypes and provide a model system for drug testing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Atherosclerosis*
Cell Line
Diabetes Mellitus, Type 2*
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Twins, Monozygotic