Generation of the induced pluripotent stem cell line UHOMi002-A from peripheral blood mononuclear cells of a healthy male donor

Mathieu Fieldes; Engi Ahmed; Chloé Bourguignon; Joffrey Mianné; Mélanie Martin; Cécile Arnould; Isabelle Vachier; Said Assou; John De Vos; Arnaud Bourdin

doi:10.1016/j.scr.2020.102037

Generation of the induced pluripotent stem cell line UHOMi002-A from peripheral blood mononuclear cells of a healthy male donor

Stem Cell Res. 2020 Dec:49:102037. doi: 10.1016/j.scr.2020.102037. Epub 2020 Oct 8.

Authors

Affiliations

¹ IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France.
² IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France; Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France.
³ Laboratory of Molecular Genetics, Univ Montpellier, CHU Nîmes, Nîmes, France.
⁴ Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France.
⁵ IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France; Department of Cell and Tissue Engineering, Saint-Eloi Hospital, Montpellier 34000, France. Electronic address: [email protected].
⁶ Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France; PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214. Electronic address: [email protected].

PMID: 33099111
DOI: 10.1016/j.scr.2020.102037

Abstract

Human pluripotent stem cells (hiPSC) are highly valuable tools to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized 40-year-old healthy male nonsmoking donor. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai Virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers. The reported UHOMi002-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing.

MeSH terms

Adult
Cell Differentiation
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells*
Karyotype
Leukocytes, Mononuclear
Male
Transcription Factors / genetics

Substances

Transcription Factors