Generation of Pancreatic Organoid-Derived Isografts

Sabrina D'Agosto; Francesca Lupo; Vincenzo Corbo

doi:10.1016/j.xpro.2020.100047

Generation of Pancreatic Organoid-Derived Isografts

STAR Protoc. 2020 Jun 6;1(2):100047. doi: 10.1016/j.xpro.2020.100047. eCollection 2020 Sep 18.

Authors

Sabrina D'Agosto¹, Francesca Lupo¹, Vincenzo Corbo¹

Affiliation

¹ Department of Diagnostics and Public Health, University of Verona, Verona 37134, Italy.

Abstract

This protocol is a procedure for generating orthotopic isografts using mouse pancreatic cancer organoids. These isografts can be used to track the evolution of pancreatic ductal adenocarcinoma (PDA) from a preinvasive lesion to a metastatic disease and therefore represent a suitable model for identification of determinants of PDA progression. For complete details on the use and execution of this protocol, please refer to Boj et al. (2015) and Filippini et al. (2019).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Disease Progression
Isografts / cytology*
Mice
Organoids / cytology*
Pancreatic Neoplasms / pathology*
Tumor Cells, Cultured / cytology*