Moringa oleifera Lam. (MO) leaf powder has been well studied, however, understanding how extraction methods of antioxidant compounds affect human primary fibroblasts still needs to be determined. The antioxidant capacity was analyzed through a copper reduction capacity method and primary human skin fibroblasts were evaluated for cytotoxicity using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Antioxidant activity under the influence of methanolic solvents (Trolox equivalents of 160.18 nmol/µL) was 17 times higher than under aqueous solvents. Interestingly, the aqueous extract showed less toxicity in comparison to the methanolic, as cells resulted more susceptible to concentrations ranging from 0.05 to 5 mg/L. Although, MO methanol solvent showed a higher antioxidant capacity in comparison to the aqueous solvent, it presented greater cytotoxicity. Thus, it is concluded that the aqueous extract could be suitable for downstream processing and applications.
Keywords: MTT Assay; Moringa oleifera Lam.; antioxidant activity; cytotoxicity; fibroplasts.