Application of TiO₂ nanoparticles to reduce bioaccumulation of arsenic in rice seedlings (Oryza sativa L.): A mechanistic study

The possible application of TiO₂ nanoparticles (nano-TiO₂) to alleviate arsenic bioaccumulation in rice seedlings and such a functioning with their crystalline structure were investigated. Specifically, nano-TiO₂ with anatase and rutile structures and the bulk TiO₂ at 0, 10, 100, and 1000 mg/L were amended to the hydroponic exposure systems with arsenic concentration at 1 mg/L, and the plant was exposed for 7 days. Our findings indicated that nano-TiO₂ significantly reduced arsenic bioaccumulation in rice seedlings by 40-90% via strong sorption process, but their growth was not affected. Nano-TiO₂ amendment notably alleviated oxidative stress resulting from arsenic exposure. Without nano-TiO₂ amendment, the iron plaque on root surfaces served as a strong barrier to inhibit arsenic uptake by rice seedlings. Interestingly, nano-TiO₂ amendment significantly decreased the iron plaque amount by 50-63% and weakened the arsenic retention in this barrier by 47-99%, further verifying the overwhelming superiority of nano-TiO₂ in inhibiting arsenic uptake by rice seedlings. Rutile nano-TiO₂ (NRT) at 1000 mg/L presented to be a promising candidate for controlling arsenic uptake by the exposed rice seedlings, with no significant oxidative stress by the amended nano-TiO₂, thereby mitigating health risk of arsenic to humans via food chain.