Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system

Canwei Zhang; Yankun Yu; Fuxi Li; Xihong Lan; Li Wang

doi:10.1016/j.scr.2020.102069

Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system

Stem Cell Res. 2020 Dec:49:102069. doi: 10.1016/j.scr.2020.102069. Epub 2020 Oct 29.

Authors

Canwei Zhang¹, Yankun Yu¹, Fuxi Li¹, Xihong Lan¹, Li Wang²

Affiliations

¹ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510623, China.
² State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510623, China. Electronic address: [email protected].

PMID: 33157390
DOI: 10.1016/j.scr.2020.102069

Abstract

Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) line using the CRISPR/Cas9 genome-editing technology. We believe that this cell line will be a good resource for exploring the function of the MEIS1 gene in embryonic development in vitro. Furthermore, the gene-knockout method reported in this study is efficient and labor-saving, which may provide an effective strategy for hESC gene deletion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Line
Clustered Regularly Interspaced Short Palindromic Repeats
Embryonic Stem Cells
Human Embryonic Stem Cells*
Humans