Possible cardioprotective role of NaHS on ECG and oxidative stress markers in an unpredictable chronic mild stress model in rats

Can J Physiol Pharmacol. 2021 Mar;99(3):321-327. doi: 10.1139/cjpp-2019-0646. Epub 2020 Nov 11.

Abstract

The protective effect of H2S against various body organ injuries has been described. The aim of this work is to investigate the potential role of sodium hydrosulfide (NaHS) as an H2S donor in chronic mild stress induced changes in the rat heart. Forty adult male Sprague Dawley rats were assigned to four groups: control, stressed group, stressed rats treated with aminooxyacetic acid (AOAA), and stressed rats treated with NaHS. Arterial blood pressure (ABP) was recorded. Serum adrenaline, MDA, and GSH levels were measured. Chronic stress significantly increased HR and ABP. AOAA produced similar changes, while NaHS mitigated the rise in HR and ABP. Both stressed and AOAA-treated stressed groups showed a significant decrease in QRS amplitude and a shortening of the RR, QT, and QTc intervals with an elevation of the ST segment. NaHS produced a significant improvement in ECG recordings. Chronic stress produced a significant rise of adrenaline and MDA levels with a significant decline in GSH levels. The AOAA-treated stressed group showed similar elevations. NaHS treatment caused significant reduction in adrenaline and MDA levels but significantly improved GSH levels. In conclusion, H2S donor has a cardioprotective effect against stress-induced cardiovascular diseases through amelioration of the oxidative stress and raised adrenaline levels induced by chronic stress exposure.

Keywords: ABP; AOAA; ECG; GSH; H2S; MDA; NaHS; UCMS; acide amino-oxyacétique; adrenaline; adrénaline; stress léger imprévisible à long terme; tension artérielle.

MeSH terms

  • Animals
  • Arterial Pressure / drug effects
  • Cardiotonic Agents / therapeutic use*
  • Electrocardiography / drug effects*
  • Epinephrine / blood
  • Glutathione / blood
  • Heart Rate / drug effects
  • Male
  • Malondialdehyde / blood
  • Oxidative Stress / drug effects*
  • Rats
  • Rats, Sprague-Dawley
  • Stress, Psychological / drug therapy*
  • Sulfides / therapeutic use*

Substances

  • Cardiotonic Agents
  • Sulfides
  • Malondialdehyde
  • sodium bisulfide
  • Glutathione
  • Epinephrine