Immunological components and antioxidant activity in human milk processed by different high pressure-thermal treatments at low initial temperature and flash holding times

Food Chem. 2021 May 1:343:128546. doi: 10.1016/j.foodchem.2020.128546. Epub 2020 Nov 5.

Abstract

The effect of high pressure thermal (HPT) processing on the immunoglobulin (IgM, IgA and IgG), and cytokine content (IL-6, IL-8, IL-10, and TNF-α), and antioxidant activity of human milk was analyzed after the application of different treatments between 200, and 800 MPa at low initial temperatures (between -15, and 50 °C) and for 1 s (flash treatments). Low pressures intensities did not induce changes in Igs while at 800 MPa, all combinations reduced the control levels. IL-6 and IL-10 were not affected by any of the treatments applied while IL-8 and TNF-α were reduced at treatments which combined temperatures at 50 °C. In general antioxidant activity was not affected at the processing conditions chosen. The flash HPT treatment applied at 600 MPa and at 0 °C could be the best choice to preserve immunological parameters and the antioxidant activity of human milk.

Keywords: Antioxidant activity; Cytokine; High pressure thermal processing; Human milk; Human milk bank; Immunoglobulin; Interleukin.

MeSH terms

  • Antioxidants / chemistry*
  • Cytokines / analysis*
  • Cytokines / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Food Handling / methods
  • Humans
  • Immunoglobulins / analysis*
  • Immunoglobulins / metabolism
  • Milk, Human / chemistry
  • Milk, Human / immunology
  • Milk, Human / metabolism*
  • Pressure
  • Temperature

Substances

  • Antioxidants
  • Cytokines
  • Immunoglobulins