Delicately Designed Cancer Cell Membrane-Camouflaged Nanoparticles for Targeted 19F MR/PA/FL Imaging-Guided Photothermal Therapy

ACS Appl Mater Interfaces. 2020 Dec 23;12(51):57290-57301. doi: 10.1021/acsami.0c13865. Epub 2020 Nov 24.

Abstract

Our exploration of multimodal nanoprobes aims to combine photoacoustic (PA) imaging, 19F magnetic resonance (MR), and fluorescence (FL) imaging, which offers complementary advantages such as high spatial resolution, unlimited penetration, and high sensitivity to enable more refined images for accurate tumor diagnoses. In this research, perfluorocarbons (PFCs) and indocyanine green (ICG) are encapsulated by poly(lactic-co-glycolic acid) (PLGA) for intravital 19F MR/FL/PA tri-modal imaging-guided photothermal therapy. Then, it is coated with an A549 cancer cell membrane (AM) to fabricate versatile theranostic nanoprobes (AM-PP@ICGNPs). After systemic administration, FLI reveals time-dependent tumor homing of NPs with high sensitivity, 19F MRI provides tumor localization of NPs without background signal interference, and PAI illustrates the detailed distribution of NPs inside the tumor with high spatial resolution. What is more, AM-PP@ICGNPs accumulated in the tumor area exhibit a prominent photothermal effect (48.4 °C) under near infrared (NIR) laser irradiation and realize an enhanced antitumor response in vivo. These benefits, in combination with the excellent biocompatibility, make AM-PP@ICGNPs a potential theranostic nanoagent for accurate tumor localization and ultimately achieve superior cancer therapy.

Keywords: biomimetic nanoparticles; cancer cell membrane; homotypic targeting; photothermal tumor ablation; tri-modal imaging.

MeSH terms

  • A549 Cells
  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / radiation effects
  • Antineoplastic Agents / therapeutic use*
  • Cell Membrane / chemistry*
  • Fluorine / chemistry
  • Fluorocarbons / chemistry
  • Fluorocarbons / radiation effects
  • Fluorocarbons / therapeutic use
  • Humans
  • Indocyanine Green / chemistry
  • Indocyanine Green / radiation effects
  • Indocyanine Green / therapeutic use
  • Infrared Rays
  • Magnetic Resonance Imaging
  • Male
  • Mice, Inbred BALB C
  • Nanoparticles / chemistry
  • Nanoparticles / radiation effects
  • Nanoparticles / therapeutic use*
  • Neoplasms / diagnostic imaging
  • Neoplasms / drug therapy*
  • Optical Imaging
  • Photoacoustic Techniques
  • Photothermal Therapy / methods
  • Polylactic Acid-Polyglycolic Acid Copolymer / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer / radiation effects
  • Polylactic Acid-Polyglycolic Acid Copolymer / therapeutic use
  • Theranostic Nanomedicine / methods
  • Xenograft Model Antitumor Assays

Substances

  • Antineoplastic Agents
  • Fluorocarbons
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Fluorine
  • Indocyanine Green