Healthcare workers (HCWs) in Afghanistan faced many challenges during the COVID-19 pandemic. A cross-sectional, online survey was conducted from July 4, 2020 to July 12, 2020 to evaluate the working conditions and health situation of HCWs in Afghanistan during the pandemic. Healthcare workers from 34 provinces, who were actively working in Afghan hospitals, were invited to participate in this study; 925 HCWs completed the survey. The results showed that 85% of the HCWs participated in the study were tested positive for COVID-19. This highlights the critical need of HCWs for personal protective equipment when caring for suspected and/or confirmed cases of the COVID-19.